Journal: PLoS ONE

Article Title: Glucose-coated superparamagnetic iron oxide nanoparticles prepared by metal vapor synthesis can target GLUT1 overexpressing tumors: In vitro tests and in vivo preliminary assessment

doi: 10.1371/journal.pone.0269603

Figure Lengend Snippet: GLUT1 levels estimated by ELISA in PSN-1, BCPAP and HEK293 cells (at the top). Uptake studies: PSN-1 and BCPAP cells were incubated with 0.1 mg/mL of Glc-SPIONs for 30 min, 1, 3, 6, and 12 h. The Fe cellular content was estimated by GF-AAS analysis. Uptake studies after treating the cells with GLUT1 inhibitors: PSN1 and BCPAP cells were pre-incubated for 1 h with anti-GLUT1 polyclonal antibody, WZB117, Fasentin, BAY-876, and STF-31. Subsequently, cells were treated for 3 or 6 h with 0.1 mg/mL of Glc-SPIONs.

Article Snippet: Expression levels in cancer cells were evaluated by means of the GLUT1 Colorimetric Cell-Based ELISA kit (Boster Biological Technology, Pleasanton CA, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Incubation

Journal: Journal of Microbiology and Biotechnology

Article Title: Postbiotic Potential of Heat-Killed Lacticaseibacillus paracasei HP7 in Functional Dyspepsia

doi: 10.4014/jmb.2508.08029

Figure Lengend Snippet: Serum concentrations of ( A ) gastrin (GAS), ( B ) peptide YY (PYY), ( C ) gastric inhibitory peptide (GIP) were measured. Data are presented mean ± SD. Different letters indicate significant differences ( p < 0.05). NOR, normal group; CONT, loperamide injected group; MOS, mosapride-treated group; HP7-L, low-dose heat-killed HP7 treated group (10 8 CFU kg/day); HP7-H, high-dose heat-killed HP7 treated group (10 9 CFU/kg/day).

Article Snippet: The level of regulatory hormones such as gastrin (GAS, CSB- E12924 ; CUSABIO, USA), gastric inhibitory peptide (GIP, CSB-E08486m), and peptide YY (PYY, CSB-EL019128MO) in the serum were quantified according to the manufacturer’s instructions.

Techniques: Injection

Journal: International Journal of Molecular Sciences

Article Title: Evodiamine Induces Apoptosis and Inhibits Migration of HCT-116 Human Colorectal Cancer Cells

doi: 10.3390/ijms161126031

Figure Lengend Snippet: Migration ability, expression of PGI/AMF, JAK2/STAT3, and MMP3 were detected in HCT-116 cells treated with EVO. ( A ) Migration potential was assessed by Transwell assay after cells were treated by agents. Cells were seeded in Transwell insert upper part incubated with 10 μg/mL PGI/AMF ( b ) and with 10 μg/mL PGI/AMF + 6 μmol/L EVO ( c ) for 28 h, normal cells as the control group ( a ) (original magnification, 200×); ( B ) AMF was detected by ELISA after cells were treated with various concentrations of EVO for 6 h; ( C – F ) Cells were incubated with 6 μmol/L EVO for 2, 4, and 6 h, and the expression of PGI, MMP3 and JAK2/STAT3 was assessed. Relative expression ratio was calculated with Gray-scan value for ( D , F ). Data represent the results of three independent experiments (mean ± SD). Statistically different compared with the control (0 μmol/L of EVO) ( * p < 0.05; ** p < 0.01).

Article Snippet: The human GPI ELISA kit was purchased from Cusabio (Wuhan, China).

Techniques: Migration, Expressing, Transwell Assay, Incubation, Control, Enzyme-linked Immunosorbent Assay